Description Catalog number:EC-878Size: 1 LiterBlot in half the time versus conventional electroblotting buffersVery high-efficiency transferOptimal pH; less harsh on proteinsProtoBlot Rapid Western Blotting Buffer is a relatively low conductivity buffer designed to replace tris/glycine (Towbin) buffer in wet (tank) transfer applications. It allows protein transfer from gels to nitrocellulose or PVDF at relatively higher voltages.The speed of transfer of a protein from the gel to the membrane during a western blot is proportional to the electric field strength. It is directly proportional to the voltage applied and inversely proportional to the distance between the electrodes and is expressed in V/cm.  The amount of heat produced during the transfer is a function of the current flowing through the system. A lower conductivity buffer like ProtoBlot can be run at a higher voltage producing less heat because the current conducted will be lower. This allows for a shorter run time and more efficient transfer.Key notes:For use the 10X buffer is diluted to 1X and ethanol or methanol is added to 10%-20%.The pH of the buffer at a 1X dilution is 8.4–8.5 which is the ideal pH for maximum transfer of most proteins.The transfer can be done with or without an ice pack or cooling system.Optimum transfer times will vary and have to be determined empirically, but a suggested starting point is 300V for 20 minutes.

Description Catalog number:EC-888Size: 1 KitRecovery of All Proteins from Complex MixturesPrecipitates as Little as 100ng of BSA at 0.25µg/mlMilder than TCAMore Reliable than Acetonitrile or Ammonium SulfateThe ND Protein Precipitation Kit is mild on samples and easy to use. It casts the finest net of any procedure, allowing the high yield collection of all proteins in solution, precipitating even the most dilute proteins. The ND Protein Precipitation Kit allows collection of proteins that would be missed by other methods.Ideal for both routine and difficult work, the ND Protein Precipitation Kit stands alone among all methods. It offers the best combination of mildness, simplicity and effectiveness in protein precipitation.High Yield Recovery10ug BSA in 1ml vol and 50 ng of BSA in 200ul were prepared from stock and precipitated following kit instructions. 10ug and 50ng of BSA in 5 ul were prepared as controls. After running on a 12% gel, lanes 2 and 3 show control and recovered 10ug BSA. Lanes 5 and 6 show control and recovered 50ng BSA. Densitometry of 10ug BSA showed 91.9% recoverySimplicity of MethodReagent A and Reagent B form a coprecipitate with the protein.After a simple wash, the protein pellet is ready to be redissolved in desired buffer.

Description Catalog number:EC-899Size: 0.5 mlEngineered for Exact MobilitiesSharpest, most precise bands for electrophoresis and blottingProtoMetrics Protein Markers consist of 9 precisely sized recombinant proteins of 10, 15, 25 35, 50, 75, 100, 150, and 225 kDa.The ProtoMetrics Protein Markers are supplied in quantities of 0.5 ml per vial. Each vial contains sufficient material for 100 mini-gels.

Anti-human NKX3.1 is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant human NKX3.1 protein. The immunogen corresponds to the amino-terminal 153 amino acids of the human protein (1) . The IgG fraction was prepared by Protein A affinity chromatography. NKX3.1 is located on human chromosome 8p21.21. It is an ­androgen-­regulated homeodomain gene with the expression of the NKX3.1 protein ­predominantly localized to prostate epithelial cells (23) . Mouse nkx3.1 is an androgen-regulated homeodomain gene with the expression of Nkx3.1 protein predominantly localized to the prostate epithelial cells (5) . Anti-mouse Nkx3.1 antibody is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant mouse Nkx3.1 protein. To improve the specificity of the antibody only the N-terminal domain of mouse Nkx3.1 was produced as immunogen. The IgG fraction was prepared by protein A affinity chromatography. IgG specific to mouse Nkx3.1 was further purified using an affinity matrix produced by cross-linking the immnunogen to agarose.

Anti-human NKX3.1 is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant human NKX3.1 protein. The immunogen corresponds to the amino-terminal 153 amino acids of the human protein (1) . The IgG fraction was prepared by Protein A affinity chromatography. NKX3.1 is located on human chromosome 8p21.21. It is an ­androgen-­regulated homeodomain gene with the expression of the NKX3.1 protein ­predominantly localized to prostate epithelial cells (23) . Mouse nkx3.1 is an androgen-regulated homeodomain gene with the expression of Nkx3.1 protein predominantly localized to the prostate epithelial cells (5) . Anti-mouse Nkx3.1 antibody is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant mouse Nkx3.1 protein. To improve the specificity of the antibody only the N-terminal domain of mouse Nkx3.1 was produced as immunogen. The IgG fraction was prepared by protein A affinity chromatography. IgG specific to mouse Nkx3.1 was further purified using an affinity matrix produced by cross-linking the immnunogen to agarose.

Anti-human NKX3.1 is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant human NKX3.1 protein. The immunogen corresponds to the amino-terminal 153 amino acids of the human protein (1) . The IgG fraction was prepared by Protein A affinity chromatography. NKX3.1 is located on human chromosome 8p21.21. It is an ­androgen-­regulated homeodomain gene with the expression of the NKX3.1 protein ­predominantly localized to prostate epithelial cells (23) . Mouse nkx3.1 is an androgen-regulated homeodomain gene with the expression of Nkx3.1 protein predominantly localized to the prostate epithelial cells (5) . Anti-mouse Nkx3.1 antibody is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant mouse Nkx3.1 protein. To improve the specificity of the antibody only the N-terminal domain of mouse Nkx3.1 was produced as immunogen. The IgG fraction was prepared by protein A affinity chromatography. IgG specific to mouse Nkx3.1 was further purified using an affinity matrix produced by cross-linking the immnunogen to agarose.

Glo-Plate 2.0 Blue LED Illuminator is a multi-functional LED light box designed for photolysis of PMAxx- or PMA-treated samples in multi-well plates. It is also able to develop the staining of gels stained with the visible blue DNA gel stain DNAzure.

The PMA-Lite™ 2.0 LED Photolysis Device can be used to provide continuous illumination for up to 18 samples at a time, for convenient and controlled photoactivation of PMA, PMAxx™ , and similar dyes. The device features a touchscreen interface with easy-to-use timer.

The Gel-BrightTM Imaging Hood is a convenient light-proof covering designed for use with the Gel-BrightTM Laser Diode Gel Illuminator. The imaging hood allows users to take images of their gel during imaging with a cell phone camera or other device.

Certified to be free of DNase, RNase, and endotoxins (pyrogens). Manufactured under a strict control in an ISO 9001 certified facility. Every lot is tested for engineering compliance and accuracy. All raw materials are traceable.

Certified to be free of DNase, RNase, and endotoxins (pyrogens). Manufactured under a strict control in an ISO 9001 certified facility. Every lot is tested for engineering compliance and accuracy. All raw materials are traceable.

Certified to be free of DNase, RNase, and endotoxins (pyrogens). Manufactured under a strict control in an ISO 9001 certified facility. Every lot is tested for engineering compliance and accuracy. All raw materials are traceable.

For quantitative determination of ACE-1 activity determination in biological samples. Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Sensitive and accurate. Linear detection range 0.01 – 5 U/L ACE-1 in a 96-well plate assay. High-throughput. Can be readily automated to assay thousands of samples per day. Method: FL360/485nm; Samples: Serum, cell or tissue lysate, and other biological samples; Procedures: 45 min

Uricase activity in biological samples such as cell culture, non-human biofluids, cell extracts, and plant tissue extracts. Sensitive. Use 10 μL samples. Linear detection range: fluorimetric assay 0.3 – 100 U/L Uricase. Fast. Run time is 10 minutes for rapid results. Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day. Method: FL530/585nm; Samples: Biological samples; Species: All (except hominids) ; Procedures: 10 min

Uric acid determination in biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts. Sensitive. Use 10 µ L samples. Linear detection range: Colorimetric assay 15 – 1000 µ M Uric Acid. Fluorimetric assay 4 – 300 µ M Uric Acid. Fast. Run time is 30 minutes for Optical Density method and 10 minutes for Fluorimetric method for rapid results. Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day. Method: OD570 or FL530/585nm; Samples: Biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts

This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma. [provided by RefSeq, Apr 2009]

The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq, Jul 2008]

Horseradish Peroxidase Conjugated Goat Polyclonal Antibody to Human a-1-Antitrypsin, 1mL With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.

Colloidal Gold Conjugated Monoclonal Antibody to Tubulin, 10nm With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.

Gal b(1,3)GalNac b-O-BSA Colloidal Gold Conjugate, 1mL, available in 5-40nm particle sizes (please select above). Additional sizes available upon request, surcharges will apply. With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le (x) antigen exhibit strong self-aggregation, based on calcium-dependent Le (x) -Le (x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le (x) and its isomer sialyl-Le (a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Buccutite™ Rapid Oligo Antibody Conjugation Kit is optimized for conjugating 100 ug protein with an amino-modified oligo.

This Benzoyl-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate used for the detection of enzymatic activity of human alpha-thrombin.

Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.

TheDetectX® Thromboxane B2 (TXB2) ELISA Kitquantitatively measures TXB2present in a variety of samples.

TheDetectX® Thromboxane B2 (TXB2) ELISA Kitquantitatively measures TXB2present in a variety of samples.

Please note that as of January 1, 2021, this product will no longer be available. Please seecatalog number K062-Hinstead.

Recombinant SARS-CoV-2 Spike protein, RBD (Receptor Binding Domain) encompassing amino acids 319-541. The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis) . The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.

Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 16-685. This protein corresponds to SARS-CoV-2 Variant B.1.1.7, also known as variant Alpha, originally identified in the United Kingdom. It contains mutations N501Y, A570D, D614G, P681H, and deletions 69-70HV and 144Y. The construct also contains a C-terminal Avi-Tag™ followed by a C-terminal His-tag (6xHis) . The recombinant protein was affinity purified.

Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells. BAFF has been demonstrated to activate the survival of B-cells and the B-cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.

BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells. BAFF has been demonstrated to activate the survival of B-cells and the B-cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.

BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells. BAFF has been demonstrated to activate the survival of B-cells and the B-cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.

Plant Preservative Mixture (PPM™), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne, and endogenous contamination. Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.

Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium. The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.

Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium. The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.

Biotinylated Rhesus macaque Mamu-A*08&B2M Monomer Protein (MAM-R82E5) is expressed from human 293 cells (HEK293) . It contains AA Gly 17 - Pro 299 (Mamu-A*08) & Ile 21 - Met 119 (B2M) (Accession # Q9MW40 (Mamu-A*08) & Q6V7J5 (B2M) ) .

Inositol 1,4,5-triphosphate receptor interacting protein like 1 (ITPRIPL1) is a single transmembrane protein, exhibits normal testis-enriched expression and widespread overexpression in various human cancers. ITPRIPL1 is commonly found in tumors with low PD-L1 expression. ITPRIPL1 acts as a natural ligand for CD3 ε, evading immune surveillance, leading to decreased T cell activity and promoting tumor growth. Research has shown that inducing stable CD3E-NCK1 binding prevents CD3E-ZAP70 interaction, subsequently inhibiting downstream ERK-NFkB signaling cascade and calcium influx activation.

Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD) . Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

Toll like receptor 8, also known as CD288, is a member of TLR family. It plays an important role in sensing phagocytosed pathogen. TLR8 recognizes ssRNA and recruits MyD88 and leads to the activation of transcription factor NF-kappa B. TLR8 TLR8 has two splice variants, viz., TLR8v1 and TLR8v2 and one missense variant, termed G or A1G allele of TLR8. TLR8v1 has additional 19 amino acids in the N-terminus. It is predominantly expressed in lung and peripheral blood leukocytes.

Toll like receptor 8, also known as CD288, is a member of TLR family. It plays an important role in sensing phagocytosed pathogen. TLR8 recognizes ssRNA and recruits MyD88 and leads to the activation of transcription factor NF-kappa B. TLR8 TLR8 has two splice variants, viz., TLR8v1 and TLR8v2 and one missense variant, termed G or A1G allele of TLR8. TLR8v1 has additional 19 amino acids in the N-terminus. It is predominantly expressed in lung and peripheral blood leukocytes.

Toll like receptor 8, also known as CD288, is a member of TLR family. It plays an important role in sensing phagocytosed pathogen. TLR8 recognizes ssRNA and recruits MyD88 and leads to the activation of transcription factor NF-kappa B. TLR8 TLR8 has two splice variants, viz., TLR8v1 and TLR8v2 and one missense variant, termed G or A1G allele of TLR8. TLR8v1 has additional 19 amino acids in the N-terminus. It is predominantly expressed in lung and peripheral blood leukocytes.

This is a FITC-Mouse Anti-Human IgG (H+L) Secondary antibody from Reddot Biotech. This product is for Research Use Only.

This is a AF405-Goat Anti-Rat IgG (H+L) Secondary antibody from Reddot Biotech. This product is for Research Use Only.

This is a FITC-Goat Anti-Mouse IgG F (ab') 2 Secondary antibody from Reddot Biotech. This product is for Research Use Only.

The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving: Next Generation Sequencing PCR Transfection Western blotting Immunoassays The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers. Intuitive Usability Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory. All data can also be exported easily to a PC using a USB flash drive. Accurate Quantification The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements. The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations. The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits. Options for Throughput The Smart-Q portfolio includes two distinct models, each designed to meet specific laboratory requirements: The Smart-Q 100 allows for single-sample processing in 0.5mL assay tubes (NS1020-T) . This renders the Smart-Q 100 ideal for laboratories processing fewer samples. The Smart-Q 800 caters to high-throughput workflows and allows for up to eight samples to be evaluated at once using individual 0.2mL tubes or 8 x 0.2mL strips tubes (Order no. F1001-T and F1001-T-8, ) . The use of a standard 8-channel pipettor can streamline both the reagent preparation and sample transfer, making the Smart-Q 800 an efficient solution for workflows requiring parallel processing of multiple samples. Accuris DNA Quantification Kits Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using an instrument such as the SmartDrop XF, Qubit™or Accuris™ Fluorometer. The reagents are highly selective for double-stranded DNA (dsDNA), and not for single-stranded DNA (ssDNA), RNA, protein, or nucleotides. Included with each kit are a concentrated assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL of sample is needed), and the concentration can be determined using an appropriate fluorometer instrument. High Sensitivity dsDNA Assay Kit The Accuris High Sensitivity kit provides an accurate method for quantitation of dsDNA samples when sensitivity is required. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/µL, providing a detection range of 0.1−120 ng. 0.1 to 100 ng/ul, providing detection of 0.1 to 100 ng Broad Range dsDNA Assay Kit The Accuris Broad Range kit provides an accurate method for quantitation of dsDNA samples for a broad range quantity of DNA. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng.

The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving: Next Generation Sequencing PCR Transfection Western blotting Immunoassays The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers. Intuitive Usability Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory. All data can also be exported easily to a PC using a USB flash drive. Accurate Quantification The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements. The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations. The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits. Options for Throughput The Smart-Q portfolio includes two distinct models, each designed to meet specific laboratory requirements: The Smart-Q 100 allows for single-sample processing in 0.5mL assay tubes (NS1020-T) . This renders the Smart-Q 100 ideal for laboratories processing fewer samples. The Smart-Q 800 caters to high-throughput workflows and allows for up to eight samples to be evaluated at once using individual 0.2mL tubes or 8 x 0.2mL strips tubes (Order no. F1001-T and F1001-T-8, ) . The use of a standard 8-channel pipettor can streamline both the reagent preparation and sample transfer, making the Smart-Q 800 an efficient solution for workflows requiring parallel processing of multiple samples. Accuris DNA Quantification Kits Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using an instrument such as the SmartDrop XF, Qubit™or Accuris™ Fluorometer. The reagents are highly selective for double-stranded DNA (dsDNA), and not for single-stranded DNA (ssDNA), RNA, protein, or nucleotides. Included with each kit are a concentrated assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL of sample is needed), and the concentration can be determined using an appropriate fluorometer instrument. High Sensitivity dsDNA Assay Kit The Accuris High Sensitivity kit provides an accurate method for quantitation of dsDNA samples when sensitivity is required. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/µL, providing a detection range of 0.1−120 ng. 0.1 to 100 ng/ul, providing detection of 0.1 to 100 ng Broad Range dsDNA Assay Kit The Accuris Broad Range kit provides an accurate method for quantitation of dsDNA samples for a broad range quantity of DNA. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng.

The Benchmark HS-10 Plus Hotplate Stirrer supports heating and stirring of up to five sample vessels, simultaneously. Unlike traditional multi-position stirrers, the HS-10 model features a large (10 x 10 in.) square top plate that provides the flexibility to heat and stir larger sample vessles, up to ten inches in diameter (including 1x10L bottles) . The top-plate features a durable, chemically resistant, white-ceramic coating that provides exceptionally uniform heating for temperature up to 400°C. The five stirring locations are clearly marked, to ease sample loading and ensuring proper positioning of vessels. Temperature and mixing speed can be viewed on the large digital display and a safety LED illuminates when the top plate temperature exceeds 50°C. An external temperature probe can also be used for applications that require precise control of sample temperature. The probe provides direct feedback to the microprocessor with constant monitoring and adjustments for maintaining the selected temperature in the sample within +/- 1°C.

PEGylation reagent for "Click" Chemistry. This PEG crosslinking reagent with a terminal alkyne functionality on one chain end reacts with azides under "Click" Chemistry conditions (for example Cu (I) catalyzed alkyne-azide cycloaddition, CuAAC) to form a stable triazole link between the two reaction partners. PEG-NHS derivatives are active esters and useful reagents to pegylate proteines, peptides or any other surfaces with a free primary amino group to form stable amide bonds. The Reaction requires a neutral to alkaline pH (7 to 8.5) to proceed. However, hydrolysis competes with aminolysis of the active ester and also increases with higher pH. Thus, working with excess PEG-NHS ester is necessary to obtain high pegylation yields. The PEG butyric acid NHS ester derivative has a C4 chain directly attached to PEG. It is one of the most stable PEG NHS esters known with a hydrolysis half-life of over 20 min at pH 8. PEG crosslinkers with Boc (tert-butyloxycarbonyl) -protected amino groups on one chain end. The Boc protecting group is stable in a wide range of conditions (including highly basic environments) but may be easily removed by (strong) acids such as TFA or at low pH in water to release the primary amine. The amine functional group at one end of the PEG linker molecule allows for selective and covalent attachment to other molecules with complementary functional groups, such as carboxylic acids or activated esters, aldehydes and epoxides. The newly formed bonds are amides, imines or secondary amines. Applicable for reversible or permanent crosslinking with PEG.

PEG aldehydes are mostly amine reactive pegylation reagents. They react with free amines, peptides and proteins forming an imine bond, which can be reduced afterwards or insitu by hydrides to form a stable secondary amine bond. However, with hydrazide substrates, hydrolytically unstable hydrazone bonds are formed, thus making reversible pegylation possible.

PEG aldehydes are mostly amine reactive pegylation reagents. They react with free amines, peptides and proteins forming an imine bond, which can be reduced afterwards or insitu by hydrides to form a stable secondary amine bond. However, with hydrazide substrates, hydrolytically unstable hydrazone bonds are formed, thus making reversible pegylation possible.

Myc-DDK-tagged ORF clone for SARS-CoV-2 surface glycoprotein [Severe acute respiratory syndrome coronavirus 2], YP_009724390

Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724391. Note: ORF is codon optimized

Transient overexpression of CD8A, transcript variant 3, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack

CD146 is a cell surface glycoprotein, originally identified as a melanoma antigen, whose expression is associated with tumor progression and the development of metastatic potential. Overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium.

Netrin receptor DCC (deleted colorectal careinoma) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. The DCC protein is a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. It is found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Native DCC is found in three isoforms. Two forms, a long and a short isoform, are produced from the same gene but have different initiation sites. The third isoform, produced by alternative splicing, is expressed only in embryonic tissue. Colorectal tumors that lost their capacity to differentiate into mucus producing cells uniformly lack DCC expression. Inactivation of DCC due to allelic deletion or point mutations may cause both lymphatic and hematogenous metastasis of esophageal squamous cell carcinomas.

CD133 antigen, also known as prominin-1, is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins, which specifically localize to cellular protrusions. When embedded in the cell membrane, the membrane topology of prominin-1 is such that the N-terminus extends into the extracellular space and the C-terminus resides in the intracellular compartment. The protein consists of five transmembrane segments, with the first and second segments and the third and fourth segments connected by intracellular loops while the second and third as well as fourth and fifth transmembrane segments are connected by extracellular loops. While the precise function of CD133 remains unknown, it has been proposed that it acts as an organizer of cell membrane topology. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, various pediatric brain tumors, as well as adult kidney, mammary glands, trachea, salivary glands, uterus, placenta, digestive tract, testes, and some other cell types.

Beta Defensin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.5kDa.

Beta Defensin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.6kDa.

Beta Defensin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 45 amino acids and having a molecular mass of 5161.2 Dalton.

Reduce endotoxin levels to 0.1 EU/µg DNA or less Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume Remove endotoxins from up to 1 mg of DNA with the Maxi format kit Fast and easy processing using a rapid spin-column format These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample. Endotoxin Removal Kit (Mini) This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes. Endotoxin Removal Kit (Midi) This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. Endotoxin Removal Kit (Maxi) This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. About Endotoxins Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Concentration of small amounts of RNA into 8 μL Ideal for concentrating RNA purified from exosomes, plasma, serum, urine, and other bodily fluids and any RNA samples initially purified in large volumes Efficient RNA cleanup from enzymatic reactions – labeling, DNase treatment and in vitro transcription Cleanup of RNA isolated using different methods, including phenol/chloroform extractions Fast and easy processing using rapid spin-column format Suitable for all sizes of RNA, from large rRNA down to microRNA (miRNA) No phenol or chloroform extractions Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix. Norgen’s EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) . The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.

Extract high quality & quantity total RNA including miRNA Isolate from a wide variety of animal tissues No phenol step required - isolate all RNA in one fraction Compatible with tissues stored in RNAlater® or Trizol® Convenient & fast spin column format Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc. Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more. The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

A Calcein AM efflux assay to screen a wide range of compounds for multidrug resistance transporter activity Excitation: 528 nm | Emission: 617 nm Calcein AM is a membrane-permeant, non-fluorescent dye that enters cells passively. Once inside the cytosol of cells, intracellular esterases convert it to fluorescent Calcein (Ex/Em: 495 nm/515 nm), resulting in uniform cytosolic fluorescence. Drug efflux transporters, such as P-glycoprotein (Pgp, MDR1) and multidrug-resistance-associated protein (MRP1), actively extrude Calcein AM from inside the cell before esterases can convert it to Calcein. The presence of additional MDR substrates or inhibitors of MDR expression results in decreased Calcein AM efflux, causing a measurable increase in intracellular fluorescence. In addition to identifying MDR substrates and inhibitors, this kit can also be used to evaluate the activity of MDR transporters in cells. When following our protocol, ION’s MDR Activity kit provides enough reagents to make 100 mL of working solution, enough for ten 96- or 384-well plates or 80 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.

Calcium indicator, membrane impermeable. Compatible with flow cytometry, plate reader, and fluorescence microscopy assays. Excitation: 490 nm | Emission: 515 nm | Kd: 355 nM | Purity: >95% Fluo-4 is the most used and the most trusted of the single-wavelength Ca²⁺ indicators. It has been the indicator of choice for countless basic research studies and has been the used to screen many tens-of-millions of compounds using high-throughput screening (HTS) to discover pharmacological tools and drugs for GPCRs, and a wide variety of Ca²⁺ permeable ion channels and transporters. Fluo-4 K⁺ Salt is a membrane impermeable form of Fluo-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.

Calcium indicator, membrane permeable. Compatible with flow cytometry, plate reader, and fluorescence microscopy assays. Excitation: 490 nm | Emission: 515 nm | Kd: 355 nM | Purity: >95% Fluo-4 is the most used and the most trusted of the single-wavelength Ca²⁺ indicators. It has been the indicator of choice for countless basic research studies and has been the used to screen many tens-of-millions of compounds using high-throughput screening (HTS) to discover pharmacological tools and drugs for GPCRs, and a wide variety of Ca²⁺ permeable ion channels and transporters.

Our Optitrol NAT controls match all commonly used assays for infectious disease testing.They are registered IVDs, manufactured in an ISO 13485 certified facility, and are standardized against the WHO international standard.All NAT controls come with a shelf-life of 24 months at a storage temperature of -20°C for the closed vial.The vials are barcoded and fit directly into your instrument.One vial of 1.2 ml is sufficient for one run.

Our Optitrol NAT controls match all commonly used assays for infectious disease testing.They are registered IVDs, manufactured in an ISO 13485 certified facility, and are standardized against the WHO international standard.All NAT controls come with a shelf-life of 24 months at a storage temperature of -20°C for the closed vial.The vials are barcoded and fit directly into your instrument.One vial of 1.2 ml is sufficient for one run.

Our Optitrol NAT controls match all commonly used assays for infectious disease testing.They are registered IVDs, manufactured in an ISO 13485 certified facility, and are standardized against the WHO international standard.All NAT controls come with a shelf-life of 24 months at a storage temperature of -20°C for the closed vial.The vials are barcoded and fit directly into your instrument.One vial of 1.2 ml is sufficient for one run.

- With reservation and Timer functions, there is no need to wait, which effectively improves the efficiency of the experiment - Available in Chinese and English menus to meet different language needs, with °C / °F conversion - The height of the shelf is adjustable to meet different cultivation needs - Professional stacking foot design, the machine can be stacked, saving lab space and improving the use efficiency

- With reservation and Timer functions, there is no need to wait, which effectively improves the efficiency of the experiment - Available in Chinese and English menus to meet different language needs, with °C / °F conversion - The height of the shelf is adjustable to meet different cultivation needs - Professional stacking foot design, the machine can be stacked, saving lab space and improving the use efficiency

This sturdy, reliable mixers can adjust speed to meet different experimental requests. Multiple mixing modes, Touch and continuous operations. Aluminium cast base avoids unnecessary “walking”. A variety of optional adapters are available which process 70 samples at the same time to make the experiment more convenient and efficient. Wide speed range:0-3000rpm. Built-in short mix, Microprocessor control, LED display the speed and time. - Exquisite appearance and sturdy construction; Special machinefoot adsorption desktop, super stable and shockproof - Stepper motor with stable performance, high power torque, low noise, stable low speed, strong high speed and long service life - The three-inch color screen hologram displays all the parameters, graphical displays the current running attitude mode of the test tube, which is clear at a glance, and sound an alarm after the end of the operation - Built-in M1~M10 ten kinds of mixing mode, one key to call, convenient and fast, to meet more experimental needs