Research use
For laboratory applications
Worldwide delivery
International shipping available
Technical information
Detailed specifications included
Human recombinant BAFF (B-cell activating factor) protein, AF
Catalog:0519-PROTQ9Y275-6-5ug
Available sizes
1 optionAvailability
Stock products usually ship within 24–48 hours. Non-stock products may require approximately 2–6 weeks.
Shipping conditions
No dry-ice requirement is indicated for this product.
Hazardous classification
This product is not currently marked as hazardous.
BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells. BAFF has been demonstrated to activate the survival of B-cells and the B-cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.
This product is intended for laboratory and research use only. Review the supplied documentation before use.
Product Name
Human recombinant BAFF (B-cell activating factor) protein, AF
Catalog Number
0519-PROTQ9Y275-6-5ug
Available Sizes
5 µg
Hazardous
No
Dry Ice
No
Datasheet
https://www.bosterbio.com/datasheet?sku=PROTQ9Y275-6
Storage Conditions
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Form
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Reactivity
Human
Applications
Cell Culture
Gene Name
TNFSF13B
Purification
>98% as determined by SDS-PAGE.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Uniprot
Q9Y275
Aa Sequence
MAVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL with polyhistidine tag at the C-terminus.
Source
Escherichia coli
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Bioactivity
Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <0.5 ng/mL.
Tag
His Tag (C-term)
Synonyms
TNFSF13B, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4
Molecular Weight
The protein has a calculated MW of 17.98 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis) .