Fluoroshield with DAPI & DABCO

Product Name

Fluoroshield with DAPI & DABCO

Catalog Number

0436-AR-6505-01

Available Sizes

20 mL

Hazardous

No

Dry Ice

No

Applications

IHC

Assay Protocol

1. Bring the vial to room temperature. 2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light. 3. Turn the vial upside down and open the dropper to remove any air bubbles. 4. Apply 2-3 drops of mounting medium directly on top of the specimen. 5. Let stand at room temperature for about 3-5 minutes in the dark. 6. Apply cover slip, carefully avoiding air bubbles. 7. The specimen is ready for visualization under a microscope. 8. One can seal the edges of the cover slip with nail polish, any organic mounting medium or our Organo mounting medium (cat. # AR-6504) . If a coverslip is not sealed, air bubbles will appear in few days. 9. Method for applying Coverslip: Put 1-2 drops of FLS on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. Incubate at 37C for one hour in the dark to dry organic mounting medium. 10. For long term storage it is recommended that the slide be stored in the dark at 2-8oC.

Cell Type

rat brain

Detection Method

Immunofluorescence, confocal microscopy

Dilution

1:500

Label

ICT

Other Statements

For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Specifications

Ready to use mounting medium

Storage Temperature

Room temperature; Long term storage 2-8 °C recommended; Protect from light; DO NOT FREEZE

Target Description

Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phycocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. May encounter problems with FROZEN BRAIN OR OTHER FROZEN TISSUES WITH LOTS OF FAT

Type

IHC Reagents

Wavelength

360nm/460nm

Certification

RUO

Background

Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phycocyanin (PC), and Allophycocyanin (APC) . Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. May encounter problems with FROZEN BRAIN OR OTHER FROZEN TISSUES WITH LOTS OF FAT

Conjugate Molar Ratio

360nm/460nm

Method

Immunofluorescence, confocal microscopy

Storage Conditions

Room temp; Long term storage 2-8 °C recommended; Protect from light; DO NOT FREEZE